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Beuuveriu hsim, pwn in potato dextrose broth and measured their activity by their ability to agglutinate human erythrocytes. The lectins were purified by ion-exchange chromatography and affinity methods to give preparations that gave a single band in SDS-PAGE. Basic characterization has been carried out. The X. polvtnorphu lectin shows specificity for lactose and the B. hassiunu lectin for N-acetyl-Dglucosamine; both lectins have subunit masses of approx. 17 kDa. The N-terminal of each protein was determined. The X. pnlvmrphu lectin shows similarities with the galectin isolated from the fungus Coprims cinereus and is, possibly, a new member of the galectin family. B. hassiaM is insecticidal and it is interesting to note that its lectin has sequence homology with the N-acetyl-D-glucosamine-specific lectin from the fungus

Background: HCV RT-(Nested) PCR strategies arc used to study evolution at certain important viral genetic loci, predominantly at the Hypervariable Region (HVR) of the E2 envelope gene, and the lntcrferon Sensitivity Determining Region (ISDR) of the NSSa gene. We have found that the choice of DNA polymerase employed in viral PCR has profound effects on the infemd viral diversity at two distinct loci on the HCV genome. Methods: Nested HVR and ISDR PCR was performed with both proofreading (Rvo) DNA polymerase and non-proofreading (Taq) DNA polymerase on identical cDNA derived from three separate HCV positive sera. Amplimns wm cloned and sequences determined for 18-20 individual clones per sample. Results: Quasi species diversities determined from HVR and ISDR FCR products showed that there was a profound effect on the infed diversity depending on which polymerase was employed in …