1. Evidence of cross-reactivity between porcine pancreatic and rapeseed (Brassica napus L.) lipases
- Author
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Jeannette Ben Hamida, Taoufik Jridi, and Hatem Belguith
- Subjects
Rapeseed ,Swine ,Triacylglycerol lipase ,Enzyme-Linked Immunosorbent Assay ,Brassica ,Biology ,Cross Reactions ,medicine.disease_cause ,Biochemistry ,Cross-reactivity ,Epitope ,Antigen-Antibody Reactions ,medicine ,Animals ,Lipase ,Pancreas ,Differential centrifugation ,chemistry.chemical_classification ,Antiserum ,Kinetics ,Enzyme ,chemistry ,biology.protein ,Cotyledon - Abstract
A cross-reactivity between animal and plant lipases was determined, using immunological techniques. It was shown by ELISA and dot-blotting that these antibodies react with lipases in the rapeseed crude extract and in the different cellular fractions obtained by differential centrifugation. Pre-incubation of the antiserum with the rapeseed crude extract affects the amount of antibodies binding to the porcine pancreatic lipase. Antibodies were able to precipitate lipase activity from 3-day-old rapeseed crude extract. These epitopes seem to be located in the catalytic site, suggesting that a consensus sequence exists in oleaginous lipases and that it will be universal.
- Published
- 2001