1. Rapid S-nitrosothiol metabolism by platelets and megakaryocytes
- Author
-
Michael P. Gordge, Ian C. Locke, Chirag M. Shah, and Hardial S. Chowdrey
- Subjects
Blood Platelets ,S-Nitrosothiols ,Serum Albumin, Bovine ,Biological activity ,Metabolism ,Biology ,Glutathione ,Biochemistry ,Cell Line ,Nitric oxide ,chemistry.chemical_compound ,medicine.anatomical_structure ,chemistry ,Megakaryocyte ,Cell surface receptor ,medicine ,Extracellular ,Biophysics ,Humans ,Platelet ,Megakaryocytes ,Acivicin ,Nitroso Compounds - Abstract
RSNOs (S-nitrosothiols) regulate platelet and megakaryocyte function, and may act in vivo as a nitric oxide reservoir. There is a discrepancy between the spontaneous rate of NO release from different RSNO compounds and their pharmacological effects, implying that target cells may mediate biological activity either by metabolism of RSNOs or by displaying cell surface receptors. In the present study, we sought evidence for rapid cell-mediated metabolism of RSNOs. Exposure of platelets to GSNO (S-nitrosoglutathione) for as little as 5 s inhibited thrombin-induced platelet aggregation by >95%; however, AlbSNO (S-nitrosoalbumin) was much less effective over these short time periods. Incubation of 1 μM GSNO or AlbSNO with platelets and megakaryocytes resulted in a 25–34% loss of RSNO recoverable from the supernatant (P
- Published
- 2003
- Full Text
- View/download PDF