1. The role of mass spectrometry analysis in bacterial effector characterization.
- Author
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Scott NE and Hartland EL
- Subjects
- Animals, Biomedical Research trends, Gram-Negative Aerobic Bacteria pathogenicity, Gram-Negative Aerobic Bacteria physiology, Gram-Positive Bacteria pathogenicity, Gram-Positive Bacteria physiology, Host-Pathogen Interactions, Humans, Mass Spectrometry trends, Molecular Structure, Peptide Fragments chemistry, Peptide Fragments metabolism, Peptide Fragments physiology, Professional Role, Protein Processing, Post-Translational, Proteomics trends, Research Personnel, Transcription Activator-Like Effectors metabolism, Transcription Activator-Like Effectors physiology, Workforce, Biomedical Research methods, Mass Spectrometry methods, Proteomics methods, Transcription Activator-Like Effectors chemistry
- Abstract
Many secreted bacterial effector proteins play a critical role in host-pathogen interactions by mediating a variety of post-translational modifications, some of which do not occur natively within the eukaryotic proteome. The characterization of bacterial effector protein activity remains an important step to understanding the subversion of host cell biology during pathogen infection and although molecular biology and immunochemistry remain critical tools for gaining insights into bacterial effector functions, increasingly mass spectrometry (MS) and proteomic approaches are also playing an indispensable role. The focus of this editorial is to highlight the strengths of specific MS approaches and their utility for the characterization of bacterial effector activity. With the capability of new generation MS instrumentation, MS-based technologies can provide information that is inaccessible using traditional molecular or immunochemical approaches., (© 2017 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.)
- Published
- 2017
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