1. GDI-1 preferably interacts with Rab10 in insulin-stimulated GLUT4 translocation
- Author
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Jinzhong Zhang, Yu Chen, Yongqiang Deng, Lu Yang, Tao Xu, and Xiangyang Xie
- Subjects
Immunoprecipitation ,medicine.medical_treatment ,adipocytes ,Chromosomal translocation ,guanine-nucleotide-dissociation inhibitor (GDI) ,Plasma protein binding ,BFA, brefeldin A ,Biochemistry ,FRET, fluorescence resonance energy transfer ,Cell Line ,GAP, GTPase-activating protein ,Mice ,TIRFM, total internal reflection microscopy ,medicine ,Animals ,Humans ,Insulin ,mKO, monomeric Kushibara Orange ,Molecular Biology ,Guanine Nucleotide Dissociation Inhibitors ,GLUT4, glucose transporter 4 ,HA, haemagglutinin ,Glucose Transporter Type 4 ,biology ,GSV, GLUT4 storage vesicle ,TGN, trans-Golgi network ,Glucose transporter ,GDI, guanine-nucleotide-dissociation inhibitor ,Cell Biology ,EGFP, enhanced green fluorescent protein ,Transport protein ,Cell biology ,Protein Transport ,Förster resonance energy transfer ,glucose transporter 4 (GLUT4) ,rab GTP-Binding Proteins ,total internal reflection microscopy (TIRFM) ,HEK-293 cell, human embryonic kidney 293 cell ,fluorescence resonance energy transfer (FRET) ,Rab10 ,PM, plasma membrane ,biology.protein ,NIH 3T3 Cells ,GLUT4 ,hormones, hormone substitutes, and hormone antagonists ,Research Article ,Protein Binding - Abstract
Insulin stimulates GLUT4 (glucose transporter 4) translocation in adipocytes and muscles. An emerging picture is that Rab10 could bridge the gap between the insulin signalling cascade and GLUT4 translocation in adipocytes. In the present study, two potential effectors of Rab10, GDI (guanine-nucleotide-dissociation inhibitor)-1 and GDI-2, are characterized in respect to their roles in insulin-stimulated GLUT4 translocation. It is shown that both GDI-1 and GDI-2 exhibit similar distribution to GLUT4 and Rab10 at the TGN (trans-Golgi network) and periphery structures. Meanwhile, GDI-1 clearly interacts with Rab10 with higher affinity, as shown by both immunoprecipitation and in vivo FRET (fluorescence resonance energy transfer). In addition, the participation of GDIs in GLUT4 translocation is illustrated when overexpression of either GDI inhibits insulin-stimulated GLUT4 translocation in 3T3-L1 adipocytes. Taken together, we propose that GDI-1 is preferentially involved in insulin-stimulated GLUT4 translocation through facilitating Rab10 recycling.
- Published
- 2009