1. Glycosylation at Asn91 of H1N1 haemagglutinin affects binding to glycan receptors
- Author
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Karthik Viswanathan, Akila Jayaraman, Zachary Shriver, Jing Li, Ram Sasisekharan, Xiaoying Koh, and Rahul Raman
- Subjects
Glycosylation ,viruses ,Hemagglutinin Glycoproteins, Influenza Virus ,Biochemistry ,influenza virus ,Protein Structure, Secondary ,chemistry.chemical_compound ,N-linked glycosylation ,MS/MS, tandem MS ,SIN, Significant Interaction Network ,chemistry.chemical_classification ,HA, haemagglutinin ,0303 health sciences ,H1N1 ,030302 biochemistry & molecular biology ,HRP, horseradish peroxidase ,GlcNAc, N-acetylglucosamine ,MALDI, matrix-assisted laser-desorption ionization ,3. Good health ,RBS, receptor-binding site ,Viral evolution ,Host adaptation ,Asparagine ,Research Article ,Protein Binding ,Glycan ,Molecular Sequence Data ,haemagglutinin ,Biology ,03 medical and health sciences ,Polysaccharides ,TOF/TOF, tandem time-of-flight ,Humans ,Amino Acid Sequence ,Molecular Biology ,030304 developmental biology ,glycan receptor ,Cell Biology ,Viral membrane ,Neu5Ac, N-acetylneuraminic acid ,Protein Structure, Tertiary ,Gal, galactose ,LN, type 2 lactosamine repeat unit ,carbohydrates (lipids) ,chemistry ,Viral replication ,biology.protein ,S, sialyl group ,Glycoprotein - Abstract
The glycoprotein HA (haemagglutinin) on the surface of influenza A virus plays a central role in recognition and binding to specific host cell-surface glycan receptors and in fusion of viral membrane to the host nuclear membrane during viral replication. Given the abundance of HA on the viral surface, this protein is also the primary target for host innate and adaptive immune responses. Although addition of glycosylation sites on HA are a part of viral evolution to evade the host immune responses, there are specific glycosylation sites that are conserved during most of the evolution of the virus. In the present study, it was demonstrated that one such conserved glycosylation site at Asn91 in H1N1 HA critically governs the glycan receptor-binding specificity and hence would potentially impinge on the host adaptation of the virus.
- Published
- 2012
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