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Start Over Author "Zengin S" Journal biochemical genetics
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2. Molecular Characterization of Fusarium oxysporum f. melongenae by ISSR and RAPD Markers on Eggplant

Author

Francesco Carimi, S. Zengin, Mirko Siragusa, Ömür Baysal, Maurizio Sajeva, Emine Gümrükcü, Jaime A. Teixeira da Silva, Baysal, Ö, Siragusa, M, Gümrükcü, E, Zengin, S, Carimi, F, Sajeva, M, and Teixeira Da Silva, JA

Subjects
Genetic Markers, Fusarium, Veterinary medicine, Settore BIO/11 - Biologia Molecolare, Minisatellite Repeats, Molecular marker, Biochemistry, Fusarium, molecular marker, virulence, genetic difference, chemistry.chemical_compound, Botany, Genetic variation, Fusarium oxysporum, Genetics, Solanum melongena, DNA, Fungal, Molecular Biology, Ecology, Evolution, Behavior and Systematics, DNA Primers, Virulence, biology, Genetic Variation, food and beverages, General Medicine, biology.organism_classification, Random Amplified Polymorphic DNA Technique, RAPD, Genetic difference, chemistry, Genetic marker, Settore BIO/03 - Botanica Ambientale E Applicata, Microsatellite, Solanum
Abstract

Fusarium oxysporum f. melongenae is a major soil-borne pathogen of eggplant (Solanum melongena). ISSR and RAPD markers were used to characterize Fusarium oxysporum f. melongenae isolates collected from eggplant fields in southern Turkey. Those isolates were not pathogenic to tomato. Pathogens were identified by their morphology, and their identity was confirmed by PCR amplifi- cation using the specific primer PF02-3. The isolates were classified into groups on the basis of ISSR and RAPD fingerprints, which showed a level of genetic speci- ficity and diversity not previously identified in Fusarium oxysporum f. melongenae, suggesting that genetic differences are related to the pathogen in the Mediterranean region. The primers selected to characterize Fusarium oxysporum f. melongenae may be used to determine genetic differences and pathogen virulence. This study is the first to characterize eggplant F. oxysporum species using ISSR and RAPD.

Published
2010

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