1. DNA of Akodon (Rodentia, Cricetidae). I. Biophysical characterization
- Author
-
L. Vidal-Rioja, Liliana Semorile, and Néstor O. Bianchi
- Subjects
Base Composition ,Melting temperature ,Analytical chemistry ,Genetic Variation ,Buoyant density ,Zoology ,Rodentia ,DNA ,General Medicine ,DNA, Satellite ,Biology ,Nucleic Acid Denaturation ,biology.organism_classification ,Biochemistry ,Akodon ,Molecular Weight ,chemistry.chemical_compound ,Species Specificity ,chemistry ,Genetics ,Animals ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,Cricetidae - Abstract
Buoyant density in CsCl, melting temperature, and G + C base content of the DNA from four species of Akodon (Rodentia, Cricetidae) were determined. The buoyant density values of 1.699–1.701 g/cm3 were in accordance with the data reported for other cricetids. No satellite bands were seen in neutral CsCl. The T m values determined in 1 × SSC ranged from 86.2 to 87.0 C, which corresponds to G + C contents of 41.2–43.2%. There was good agreement in DNA base composition of the four species, although values were slightly higher in A. obscurus, suggesting a certain degree of interspecies variability.
- Published
- 1979
- Full Text
- View/download PDF