1. Role of allograft inflammatory factor-1 in bleomycin-induced lung fibrosis
- Author
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Takashi Kida, Ken Murakami, Aihiro Yamamoto, Amane Nakabayashi, Yutaka Kawahito, Masataka Kohno, Hidetake Nagahara, Y. Kukida, Kazuki Fujioka, Takahiro Seno, Takuya Inoue, Wataru Fujii, and Hiroshi Obayashi
- Subjects
0301 basic medicine ,Pulmonary Fibrosis ,Biophysics ,Bleomycin ,Biochemistry ,law.invention ,Pathogenesis ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,law ,Fibrosis ,Medicine ,Animals ,Immunologic Factors ,cardiovascular diseases ,education ,Molecular Biology ,Cells, Cultured ,education.field_of_study ,Lung ,business.industry ,Macrophages ,Calcium-Binding Proteins ,Microfilament Proteins ,Cell Biology ,respiratory system ,Macrophage Activation ,medicine.disease ,respiratory tract diseases ,Mice, Inbred C57BL ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Cell culture ,Rheumatoid arthritis ,Cancer research ,Allograft inflammatory factor 1 ,Recombinant DNA ,biological phenomena, cell phenomena, and immunity ,business - Abstract
Allograft inflammatory factor-1 (AIF-1) is a protein expressed by macrophages infiltrating the area around the coronary arteries in a rat ectopic cardiac allograft model. We previously reported that AIF-1 is associated with the pathogenesis of rheumatoid arthritis and skin fibrosis in sclerodermatous graft-versus-host disease mice. Here, we used an animal model of bleomycin-induced lung fibrosis to analyze the expression of AIF-1 and examine its function in lung fibrosis. The results showed that AIF-1 was expressed on lung tissues, specifically macrophages, from mice with bleomycin-induced lung fibrosis. Recombinant AIF-1 increased the production of TGF-β which plays crucial roles in the mechanism of fibrosis by mouse macrophage cell line RAW264.7. Recombinant AIF-1 also increased both the proliferation and migration of lung fibroblasts compared with control group. These results suggest that AIF-1 plays an important role in the mechanism underlying lung fibrosis, and may provide an attractive new therapeutic target.
- Published
- 2017