1. Analysis of allogeneic and syngeneic rat heart transplants using 23Na magnetic resonance spectroscopy.
- Author
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Waldrop SM, Alexander DZ, Lowry R, Winn KJ, Pearson TC, and Constantinidis I
- Subjects
- Animals, Heart Transplantation pathology, Magnetic Resonance Spectroscopy, Male, Organ Size, Rats, Rats, Inbred ACI, Rats, Inbred Lew, Rats, Wistar, Sodium, Time Factors, Transplantation, Homologous pathology, Transplantation, Homologous physiology, Transplantation, Isogeneic pathology, Transplantation, Isogeneic physiology, Heart Transplantation physiology
- Abstract
This study defines the total sodium-23 magnetic resonance spectroscopy (23Na MRS) signal from in vivo heterotopic rat heart transplants in the early post-transplant period and examines the utility of this noninvasive method for monitoring allograft rejection. Measurements were performed at 4.7 T. Syngeneic (n = 6) and allogeneic (n = 6) donor hearts were transplanted into the neck of recipient rats. There were 27 MRS observations between days 0 and 29 post-transplant. Heart grafts were excised at various intervals post-transplant for histologic examination. Allogeneic heart grafts rejected between days 4 and 5 post-transplant while syngeneic grafts continued to beat. All hearts showed ischemic damage. Allogeneic hearts showed cellular rejection by Day 1. 23Na MRS showed a steady elevation in signal in the 3 days prior to rejection and a sharp rise after rejection. 23Na MRS accurately identified full rejection and was also sensitive to the rejection process.
- Published
- 1996
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