1. Implications of a polyglutamine tract in the function of the human androgen receptor
- Author
-
Leen Callewaert, Annemie Haelens, Valerie Christiaens, Guy Verrijdt, Guido Verhoeven, and Frank Claessens
- Subjects
Transcriptional Activation ,SUMO-1 Protein ,Biophysics ,Biology ,In Vitro Techniques ,Biochemistry ,Transactivation ,Nuclear Receptor Coactivator 1 ,Transcription (biology) ,Coactivator ,Direct repeat ,Animals ,Humans ,Molecular Biology ,Transcription factor ,Histone Acetyltransferases ,Sequence Deletion ,Binding Sites ,Base Sequence ,Cell Biology ,Polyglutamine tract ,Molecular biology ,Recombinant Proteins ,Protein Structure, Tertiary ,Androgen receptor ,Nuclear receptor ,Receptors, Androgen ,COS Cells ,Peptides ,Plasmids ,Transcription Factors - Abstract
The androgen receptor (AR) is a ligand-dependent transcription factor and belongs to the nuclear receptor family. The AR gene contains a long polymorphic CAG repeat, coding for a polyglutamine tract. In the full size AR, the deletion of the polyglutamine tract results in an increase in the transactivation through canonical AREs. However, this effect is clearly dependent on the response elements, since it is not observed on selective elements. In our assays, a deletion of the repeat positively affected the interactions of the ligand-binding domain with the amino-terminal domain as well as the recruitment of the p160 coactivator SRC-1e to the amino-terminal domain of the AR. This is reflected by an enhanced coactivation of the AR by SRC-1e.
- Published
- 2003