1. A rolling-circle plasmid from Psychrobacter sp. TA144: evidence for a novel rep subfamily.
- Author
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Tutino ML, Duilio A, Moretti MA, Sannia G, and Marino G
- Subjects
- Amino Acid Sequence, Antarctic Regions, Bacterial Proteins genetics, Base Sequence, Cloning, Molecular, Codon, Initiator genetics, DNA, Bacterial genetics, DNA, Circular analysis, DNA, Circular genetics, DNA, Single-Stranded genetics, Gammaproteobacteria enzymology, Genetic Complementation Test, Sequence Analysis, DNA, Sequence Homology, Amino Acid, DNA Helicases genetics, DNA-Binding Proteins, Gammaproteobacteria genetics, Plasmids genetics, Trans-Activators genetics
- Abstract
In this paper we report the cloning and sequencing of two small plasmids, pTAUp and pTADw, from the Antarctic Gram-negative Psychrobacter sp strain TA144. The observation that pTAUp contains a putative Rep-coding gene (Psyrep) suggested that its duplication occurs via a rolling-circle replication mechanism. This hypothesis was confirmed by the identification of the pTAUp single-stranded DNA form. The putative pTAUp plus origin of replication was found at the 3' end of the Psyrep by using an in vivo complementation assay. Structural similarities at the level of (i) gene organization, (ii) protein sequence, and (iii) nick site sequences strongly suggest that the psychrophilic enzyme belongs to a new subfamily of replication enzymes., (Copyright 2000 Academic Press.)
- Published
- 2000
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