1. Characterization and differentiation of equine umbilical cord-derived matrix cells
- Author
-
Kathy E. Mitchell, Jeanell R. Tucker, Linda Black, Steven M. Hoynowski, Theodore Sand, Matthew T. Leming, Bryn M. Gardner, and Madeline M. Fry
- Subjects
Pathology ,medicine.medical_specialty ,Biophysics ,Clinical uses of mesenchymal stem cells ,Anthraquinones ,Biology ,Osteocytes ,Biochemistry ,Umbilical Cord ,Chondrocytes ,Osteogenesis ,Wharton's jelly ,Adipocytes ,medicine ,Animals ,Horses ,Molecular Biology ,Cells, Cultured ,Cell Proliferation ,Stem cell transplantation for articular cartilage repair ,Neurons ,Adipogenesis ,Multipotent Stem Cells ,Cell Cycle ,Mesenchymal stem cell ,Cell Differentiation ,Amniotic stem cells ,Cell Biology ,Alkaline Phosphatase ,Flow Cytometry ,Immunohistochemistry ,Multipotent Stem Cell ,Immunology ,Female ,Stem cell ,Azo Compounds ,Chondrogenesis ,Adult stem cell - Abstract
Stem cells are being evaluated in numerous human clinical trials and are commercially used in veterinary medicine to treat horses and dogs. Stem cell differentiation, homing to disease sites, growth and cytokine factor modulation, and low antigenicity contribute to their therapeutic success. Bone marrow and adipose tissue are the two most common sources of adult-derived stem cells in animals. We report on the existence of an alternative source of primitive, multipotent stem cells from the equine umbilical cord cellular matrix (Wharton's jelly). Equine umbilical cord matrix (EUCM) cells can be cultured, cryogenically preserved, and differentiated into osteo-, adipo-, chondrogenic, and neuronal cell lineages. These results identify a source of stem cells that can be non-invasively collected at birth and stored for future use in that horse or used as donor cells for treating unrelated horses.
- Published
- 2007
- Full Text
- View/download PDF