1. Gene sequences and specific detection for Panton-Valentine leukocidin
- Author
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Atsushi Fujimoto, Teruko Nakayama, Daigo Mimura, Tomoko Emura, Ikue Taneike, Saori Nakagawa, Tatsuo Yamamoto, Nobuhiro Iwakura, and Maki Kitatsuji
- Subjects
Staphylococcus aureus ,Specific detection ,Sequence analysis ,Bacterial Toxins ,Biophysics ,Leukocidin ,Virulence ,Exotoxins ,Molecular Probe Techniques ,Biology ,medicine.disease_cause ,Biochemistry ,Sensitivity and Specificity ,Microbiology ,Bacterial Proteins ,Species Specificity ,Leukocidins ,Sequence Homology, Nucleic Acid ,medicine ,Humans ,Penicillin-Binding Proteins ,Child ,Staphylococcal Protein A ,Molecular Biology ,Gene ,Reverse Transcriptase Polymerase Chain Reaction ,Chromosome Mapping ,Reproducibility of Results ,Cell Biology ,Sequence Analysis, DNA ,biochemical phenomena, metabolism, and nutrition ,Staphylococcal Infections ,bacterial infections and mycoses ,Community-Acquired Infections ,Real-time polymerase chain reaction ,Methicillin Resistance ,Panton–Valentine leukocidin ,Genome, Bacterial - Abstract
A new category of methicillin-resistant Staphylococcus aureus (MRSA), called community-acquired MRSA (CA-MRSA), has emerged worldwide. In contrast to previous MRSA, most CA-MRSA carries the Panton-Valentine leukocidin (PVL) genes (lukPVSF) as a virulence genetic trait. Sequence analysis of the lukPVSF gene of a Japanese isolate demonstrated that the gene has more similarity to methicillin-susceptible S. aureus from France than MRSA from the United States. Based on the sequences, we developed a real-time PCR assay for the three key genes of CA-MRSA; that is, lukPVSF, mecA (for methicillin resistance), and spa (for S. aureus). Dual or triple assay for lukPVSF, mecA, and spa in one test tube became possible. The detection limit of the assay with probe and SYBR Green methods was between 2.7 and 2.7 × 101 CFU/ml. The assay detected PVL-positive MRSA in clinical (blood) isolates.
- Published
- 2004