1. High expression of A-type lamin in the leading front is required for Drosophila thorax closure.
- Author
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Kosakamoto H, Fujisawa Y, Obata F, and Miura M
- Subjects
- Actins metabolism, Animals, Cell Movement, Drosophila Proteins genetics, Drosophila melanogaster cytology, Gene Knockdown Techniques, Lamins genetics, Thorax cytology, Drosophila Proteins metabolism, Drosophila melanogaster embryology, Drosophila melanogaster metabolism, Lamin Type A metabolism, Lamins metabolism, Thorax embryology
- Abstract
Tissue closure involves the coordinated unidirectional movement of a group of cells without loss of cell-cell contact. However, the molecular mechanisms controlling the tissue closure are not fully understood. Here, we demonstrate that Lamin C, the sole A-type lamin in Drosophila, contributes to the process of thorax closure in pupa. High expression of Lamin C was observed at the leading front of the migrating wing imaginal discs. Live imaging analysis revealed that knockdown of Lamin C in the thorax region affected the coordinated movement of the leading front, resulting in incomplete tissue fusion required for formation of the adult thorax. The closure defect due to knockdown of Lamin C correlated with insufficient accumulation of F-actin at the front. Our study indicates a link between A-type lamin and the cell migration behavior during tissue closure., (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Published
- 2018
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