1. Genomic structure of murine beta-1,4-galactosyltransferase
- Author
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Nancy L. Shaper, Joel H. Shaper, Gregory F. Hollis, Ilan R. Kirsch, R.J. Evans, J.M. Stafford-Hollis, and J.G. Douglas
- Subjects
Base Sequence ,Transcription, Genetic ,Molecular Sequence Data ,Restriction Mapping ,Biophysics ,Nucleic acid sequence ,Cell Biology ,Biology ,Galactosyltransferases ,Biochemistry ,Molecular biology ,Transmembrane protein ,Exon ,genomic DNA ,Mice ,Restriction map ,Coding region ,Animals ,Amino Acid Sequence ,RNA, Messenger ,Molecular Biology ,Gene ,Genomic organization - Abstract
We have isolated a series of overlapping murine genomic DNA clones that include the complete coding sequence of the Golgi membrane bound marker enzyme β-1,4-galactosyltransferase. The coding sequence is distributed into six exons spanning 50,000 b.p. of mouse chromosome 4. The COOH terminal domain is predominantly encoded by exons 2–6 and the transmembrane and amino terminal cytoplasmic domains are encoded by exon 1. S1 analysis establishes the most 5′ transcriptional initiation site 190 b.p. upstream of the first methionine residue.
- Published
- 1989