1. Evidence for Glucokinase Translocation by Glucose in Rat Hepatocytes
- Author
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Masahiro Kamiya, S. Aoki, Ogiso S, T. Nonogaki, Jun Okuda, Ichitomo Miwa, and Yukiyasu Toyoda
- Subjects
Male ,Cytoplasm ,medicine.medical_specialty ,medicine.medical_treatment ,Biophysics ,Chromosomal translocation ,Biology ,Biochemistry ,Internal medicine ,Glucokinase ,medicine ,Glucose effect ,Animals ,Insulin ,Rats, Wistar ,Molecular Biology ,Cells, Cultured ,Cell Nucleus ,Drug Synergism ,Cell Biology ,Immunohistochemistry ,Rats ,Perfusion ,Kinetics ,Subcellular distribution ,Glucose ,Endocrinology ,medicine.anatomical_structure ,Liver ,Nucleus - Abstract
By an immunohistochemical technique, we examined changes in the subcellular distribution of rat liver glucokinase in response to external stimuli. Glucokinase immunoreactivity was found predominantly in the nucleus of hepatocytes. In situ perfusion of the liver with 20 mM glucose for 10 min caused a marked decrease in nuclear immunoreactivity and an increase in cytoplasmic immunoreactivity. Insulin (10 nmol/l) potentiated this glucose effect. However, no change took place when 5 mM glucose was perfused. These results indicate that glucokinase is translocated from the nucleus to the cytoplasm in response to a high concentration of glucose.
- Published
- 1994
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