1. Adipocyte differentiation of multipotent cells established from human adipose tissue
- Author
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Christian Elabd, Anne Guezennec, Joelle Guesnet, Julien Astier, Gérard Ailhaud, Anne-Marie Rodriguez, Christian Dani, Ez-Zoubir Amri, Cécile Vernochet, Perla Saint-Marc, Frédéric Delteil, Institut de signalisation, biologie du développement et cancer (ISBDC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Yves Saint Laurent Beauté, Neuilly, France (YSL), and Yves Saint Laurent
- Subjects
Leptin ,Male ,Time Factors ,Cellular differentiation ,Cell Culture Techniques ,Adipose tissue ,Dinoprost ,Biochemistry ,Polymerase Chain Reaction ,Antioxidants ,Culture Media, Serum-Free ,chemistry.chemical_compound ,0302 clinical medicine ,Adipocyte ,MESH: Reverse Transcriptase Polymerase Chain Reaction ,Adipocytes ,MESH: Animals ,[SDV.BDD]Life Sciences [q-bio]/Development Biology ,Cells, Cultured ,0303 health sciences ,MESH: Culture Media, Conditioned ,Reverse Transcriptase Polymerase Chain Reaction ,Cell Differentiation ,MESH: Dinoprost ,MESH: Culture Media, Serum-Free ,MESH: Glucose ,Adipose Tissue ,Adipogenesis ,Adrenergic beta-1 Receptor Agonists ,030220 oncology & carcinogenesis ,Female ,Stem cell ,MESH: Adipose Tissue ,MESH: Cells, Cultured ,MESH: DNA Primers ,MESH: Cell Differentiation ,medicine.medical_specialty ,Adipose tissue macrophages ,MESH: Biological Transport ,Biophysics ,Adrenergic beta-3 Receptor Agonists ,Biology ,03 medical and health sciences ,Internal medicine ,medicine ,MESH: Blotting, Northern ,Animals ,Humans ,Masoprocol ,Cell Lineage ,Molecular Biology ,Adrenergic beta-2 Receptor Agonists ,MESH: Adipocytes ,030304 developmental biology ,DNA Primers ,MESH: Cell Culture Techniques ,MESH: Humans ,Tumor Necrosis Factor-alpha ,MESH: Time Factors ,MESH: Antioxidants ,3T3-L1 ,MESH: Polymerase Chain Reaction ,Biological Transport ,Cell Biology ,MESH: Leptin ,MESH: Cell Lineage ,Blotting, Northern ,MESH: Male ,Endocrinology ,MESH: Karyotyping ,Glucose ,chemistry ,Multipotent Stem Cell ,MESH: Tumor Necrosis Factor-alpha ,Culture Media, Conditioned ,Karyotyping ,MESH: Nordihydroguaiaretic Acid ,MESH: Receptors, Adrenergic, beta-1 ,MESH: Receptors, Adrenergic, beta-3 ,MESH: Female ,MESH: Receptors, Adrenergic, beta-2 - Abstract
International audience; In this study multipotent adipose-derived stem cells isolated from human adipose tissue (hMADS cells) were shown to differentiate into adipose cells in serum-free, chemically defined medium. During the differentiation process, hMADS cells exhibited a gene expression pattern similar to that described for rodent clonal preadipocytes and human primary preadipocytes. Differentiated cells displayed the key features of human adipocytes, i.e., expression of specific molecular markers, lipolytic response to agonists of beta-adrenoreceptors (beta2-AR agonist > beta1-AR agonist >> beta3-AR agonist) and to the atrial natriuretic peptide, insulin-stimulated glucose transport, and secretion of leptin and adiponectin. hMADS cells were able to respond to drugs as inhibition of adipocyte differentiation was observed in the presence of prostaglandin F2alpha, tumour necrosis factor-alpha, and nordihydroguaiaretic acid, a natural polyhydroxyphenolic antioxidant. Thus, for the first time, human adipose cells with normal karyotype and indefinite life span have been established. They represent a novel and valuable tool for studies of fat tissue development and metabolism.
- Published
- 2003