1. Harmine promotes molar root development via SMAD1/5/8 phosphorylation.
- Author
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Fujiwara N, Lee JW, Kumakami-Sakano M, Otsu K, Woo JT, Iseki S, and Ota MS
- Subjects
- Animals, Cell Movement drug effects, Cell Proliferation drug effects, Mice, Inbred C57BL, Molar growth & development, Molar metabolism, Smad1 Protein analysis, Smad5 Protein analysis, Smad8 Protein analysis, Tooth Root growth & development, Tooth Root metabolism, Harmine pharmacology, Molar drug effects, Phosphorylation drug effects, Smad1 Protein metabolism, Smad5 Protein metabolism, Smad8 Protein metabolism, Tooth Root drug effects
- Abstract
Tooth and bone are major tissues involved in physiological calcification in the body, and they use similar molecular pathways for development, homeostasis, and regeneration. Harmine (HMN) is a natural small compound that stimulates osteoblast differentiation in vitro and in vivo. Here we examined the biological effect of HMN on the postnatal development of molar tooth roots and periodontal tissues. HMN supported the formation of tooth roots and periodontal tissues in developing tooth germs. In tooth germ organ culture, HMN promoted the elongation of Hertwig's epithelial root sheath (HERS) and stimulated cell proliferation in HERS and dental follicle-derived tissues, including dental papillae and dental follicles. HMN stimulated cell proliferation and cell movement of HERS-derived cells without mesenchymal cells in vitro and directly induced the phosphorylation of SMAD1/5/8 protein in HERS-derived cells. Our results indicated that HMN was the first natural small compound to stimulate postnatal development of tooth germs., (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Published
- 2018
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