1. Induction of motor neuron differentiation by transduction of Olig2 protein
- Author
-
mie, masayasu, Kaneko, M., Henmi, F., and KOBATAKE, EIRY
- Subjects
Neurite ,Oligodendrocyte Transcription Factor 2 ,Neurogenesis ,Biophysics ,Nerve Tissue Proteins ,Biology ,MyoD ,Biochemistry ,Choline O-Acetyltransferase ,OLIG2 ,Mice ,Cell Line, Tumor ,medicine ,Basic Helix-Loop-Helix Transcription Factors ,Neurites ,Animals ,RNA, Messenger ,Molecular Biology ,Motor Neurons ,Cell Biology ,Motor neuron ,Molecular biology ,Cell biology ,Protein Structure, Tertiary ,Protein Transport ,medicine.anatomical_structure ,P19 cell ,nervous system ,NEUROD2 ,LHX3 - Abstract
Olig2 protein, a member of the basic helix-loop-helix transcription factor family, was introduced into the mouse embryonic carcinoma cell line P19 for induction of motor neuron differentiation. We show that Olig2 protein has the ability to permeate the cell membrane without the addition of a protein transduction domain (PTD), similar to other basic helix-loop-helix transcription factors such as MyoD and NeuroD2. Motor neuron differentiation was evaluated for the elongation of neurites and the expression of choline acetyltransferase (ChAT) mRNA, a differentiation marker of motor neurons. By addition of Olig2 protein, motor neuron differentiation was induced in P19 cells.
- Published
- 2012