1. Comparison of culture media for human intestinal organoids from the viewpoint of pharmacokinetic studies
- Author
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Hiroyuki Mizuguchi, Tatsuya Inui, Wataru Kishimoto, Jumpei Yokota, Tomoki Yamashita, Hiroshi Nakase, and Ryuga Nomoto
- Subjects
0301 basic medicine ,Duodenum ,Biophysics ,Cell Culture Techniques ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,Pharmacokinetics ,Gene expression ,medicine ,Humans ,Pharmaceutical sciences ,Molecular Biology ,Cells, Cultured ,chemistry.chemical_classification ,biology ,Intestinal organoids ,Cytochrome P450 ,Transporter ,Cell Biology ,Small intestine ,Culture Media ,Organoids ,030104 developmental biology ,Enzyme ,medicine.anatomical_structure ,chemistry ,030220 oncology & carcinogenesis ,biology.protein - Abstract
Human intestinal organoids are expected to be applied in pharmaceutical research. Various culture media for human intestinal organoids have been developed, but it remains unclear which media are preferable for pharmacokinetic studies. Here, we cultured human intestinal organoids with three major culture media that are already used widely around the world: the medium of Sato et al. (S-medium; reported in 2011), Fujii et al. (F-medium; 2018), and Miyoshi et al. (M-medium; 2013). The growth of human intestinal organoids cultured in S-medium was faster than that in F- or M-medium. The gene expression levels of most pharmacokinetic-related enzymes or transporters in human intestinal organoids cultured in M-medium were higher than those in S- or F-medium, and comparable to those in the adult human small intestine. The level of cytochrome P450 (CYP) 3A4 activity was also highest in human intestinal organoids cultured in M-medium. Collectively, the results underscored the importance of selection and optimization of culture medium for various applications using human intestinal organoids, including pharmacokinetic studies.
- Published
- 2021