1. Decursin and decursinol angelate improve wound healing by upregulating transcription of genes encoding extracellular matrix remodeling proteins, inflammatory cytokines, and growth factors in human keratinocytes
- Author
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Ngoc Anh Ho, Jeongpyo Hong, Joo-Won Suh, Jisu Han, Yoon Ju Kim, Wook Jin, Yungyeong Shin, and Hanki Lee
- Subjects
Keratinocytes ,0301 basic medicine ,Transcription, Genetic ,Biophysics ,Biochemistry ,Cell Line ,Proinflammatory cytokine ,Extracellular matrix ,03 medical and health sciences ,0302 clinical medicine ,Transcription (biology) ,Humans ,Benzopyrans ,Epidermal growth factor receptor ,Phosphorylation ,Molecular Biology ,Extracellular Matrix Proteins ,Wound Healing ,integumentary system ,biology ,Chemistry ,Cell Biology ,biology.organism_classification ,Up-Regulation ,Cell biology ,ErbB Receptors ,Butyrates ,HaCaT ,030104 developmental biology ,Angelica gigas ,030220 oncology & carcinogenesis ,biology.protein ,Cytokines ,Intercellular Signaling Peptides and Proteins ,Nanoparticles ,Emulsions ,Inflammation Mediators ,Wound healing ,Signal Transduction - Abstract
The coumarins decursin and decursinol angelate, which are found in Angelica gigas Nakai, have a variety of biological functions. Here, we show that treatment with these compounds improves wound healing by HaCaT human keratinocytes. Wound healing was increased by treatment with up to a threshold concentration of decursin, decursinol angelate, a mixture of both, and a nano-emulsion of these compounds, but inhibited by treatment with higher concentrations. Immunoblotting and fluorescence imaging of cells expressing an epidermal growth factor receptor (EGFR) biosensor demonstrated that these compounds did not stimulate wound healing by inducing EGFR phosphorylation. Rather, transcriptional analysis revealed that decursin and decursinol angelate improved wound healing by upregulating the expression of genes encoding extracellular matrix remodeling proteins, inflammatory cytokines, and growth factors.
- Published
- 2018