1. C21orf57 is a human homologue of bacterial YbeY proteins.
- Author
-
Ghosal A, Köhrer C, Babu VMP, Yamanaka K, Davies BW, Jacob AI, Ferullo DJ, Gruber CC, Vercruysse M, and Walker GC
- Subjects
- Amino Acid Sequence, Base Sequence, Conserved Sequence genetics, Molecular Sequence Data, Chloroplasts chemistry, Chloroplasts genetics, Escherichia coli Proteins chemistry, Escherichia coli Proteins genetics, Evolution, Molecular, Metalloproteins chemistry, Metalloproteins genetics, Ribonucleases chemistry, Ribonucleases genetics, Sequence Homology, Amino Acid
- Abstract
The product of the human C21orf57 (huYBEY) gene is predicted to be a homologue of the highly conserved YbeY proteins found in nearly all bacteria. We show that, like its bacterial and chloroplast counterparts, the HuYbeY protein is an RNase and that it retains sufficient function in common with bacterial YbeY proteins to partially suppress numerous aspects of the complex phenotype of an Escherichia coli ΔybeY mutant. Expression of HuYbeY in Saccharomyces cerevisiae, which lacks a YbeY homologue, results in a severe growth phenotype. This observation suggests that the function of HuYbeY in human cells is likely regulated through specific interactions with partner proteins similarly to the way YbeY is regulated in bacteria., (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Published
- 2017
- Full Text
- View/download PDF