1. Identification of genes regulating colorectal carcinogenesis by using the algorithm for diagnosing malignant state method
- Author
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Susumu Satomi, Yasushi Okazaki, Hitoshi Goto, Takehito Sakai, Shinji Takahashi, Kentaro Shimizu, Shigeki Yamaguchi, Youhei Hamaguchi, Yasuhiro Tomaru, Tomoyuki Morita, Shinji Togo, Itaru Nishizuka, Koji Kadota, Yasushi Ichikawa, Takashi Ishikawa, Shigeo Oki, Hiroyuki Nitanda, Ichiei Narita, Shugo Nakamura, Itaru Endo, Hiroshi Shimada, Hideyuki Ike, Yoshihide Hayashizaki, and Fumitake Gejyo
- Subjects
Adenoma ,Adult ,Colorectal cancer ,Biophysics ,Biology ,Malignancy ,Biochemistry ,Metastasis ,medicine ,Carcinoma ,Humans ,Molecular Biology ,Aged ,Oligonucleotide Array Sequence Analysis ,Aged, 80 and over ,Gene Expression Profiling ,Cancer ,Cell Biology ,Middle Aged ,medicine.disease ,Gene expression profiling ,Disease Progression ,DNA microarray ,Colorectal Neoplasms ,Algorithm ,Algorithms - Abstract
We studied the expression profiles of various stages of colorectal tumors (adenoma (AD), seven samples; carcinoma (CA), 16 samples) by using cDNA microarrays and developed ADMS (algorithm for diagnosing malignant state) method, selecting 335 clones characteristic of CA state. We, then, applied ADMS to 12 additional samples (five from primary lesions with metastasis and seven metastases); all 16 CAs and 12 metastatic tumors were diagnosed correctly as cancerous states. Although three of the seven ADs were diagnosed as "cancerous," the large size of two of these tumors suggested their potential malignancy. Our strategy for selecting clones characteristic of the malignant state is widely applicable to diagnosis and for predicting the stage of progression during multistep carcinogenesis. Of the 335 clones we selected, 135 were known genes. Included in the 135 genes were tumor suppressor and growth factor-related genes and were consistent with the literature. ADMS is a reliable means for identifying genes useful for the diagnosis of cancer.
- Published
- 2002
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