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Your search keyword '"Hideharu Ikebuchi"' showing total 1 results
Start Over Author "Hideharu Ikebuchi" Journal biochemical and biophysical research communications
1 results on '"Hideharu Ikebuchi"'

1. Ca2+-dependent and phorbol ester activating phosphorylation of a 36K-dalton protein of rat basophilic leukemia cell membranes and immunoprecipitation of the phosphorylated protein with IgE-anti IgE system

Author

Reiko Teshima, Hideharu Ikebuchi, and Tadao Terao

Subjects
inorganic chemicals, Immunoprecipitation, Biophysics, macromolecular substances, Immunoglobulin E, environment and public health, Biochemistry, chemistry.chemical_compound, Protein A/G, Animals, Protein phosphorylation, Amino Acids, Molecular Biology, Protein kinase C, Leukemia, biology, Cell Biology, Phosphatidylserine, Molecular biology, Phorbols, Antibodies, Anti-Idiotypic, Basophils, Neoplasm Proteins, Rats, Enzyme Activation, Molecular Weight, enzymes and coenzymes (carbohydrates), EGTA, chemistry, biology.protein, bacteria, Phosphorylation, Tetradecanoylphorbol Acetate, Calcium, Protein Kinases
Abstract

Endogeneous phosphorylation of rat basophilic leukemia cell membranes was investigated. EGTA specifically inhibited the phosphorylation of a protein having an approximate molecular weight of 36,000 dalton (36K-Da protein). Phosphorylation of this protein was enhanced by phorbol-12-myristate-13-acetate in the presence of phosphatidylserine. The phosphorylated 36K-Da protein was specifically immunoprecipitated with IgE and anti IgE anti-body. These results suggest that the phosphorylated 36K-Da protein is the β-chain of the receptor for IgE and that protein kinase C is involved in the phosphorylation mechanism.

Published
1984

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