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Your search keyword '"Frédéric Bouillaud"' showing total 3 results
Start Over Author "Frédéric Bouillaud" Journal biochemical and biophysical research communications
3 results on '"Frédéric Bouillaud"'

1. Functional Organization of the Human Uncoupling Protein-2 Gene, and Juxtaposition to the Uncoupling Protein-3 Gene

Author

Bruno Miroux, Frédéric Bouillaud, Claire Pecqueur, Anne-Marie Cassard-Doulcier, Serge Raimbault, Christophe Fleury, C. Gelly, and Daniel Ricquier

Subjects
Transcription, Genetic, Molecular Sequence Data, Biophysics, Biology, Transfection, Biochemistry, Ion Channels, Primer extension, Mitochondrial Proteins, Mice, chemistry.chemical_compound, Exon, Transcription (biology), Protein biosynthesis, Animals, Humans, Uncoupling Protein 3, Uncoupling Protein 2, Promoter Regions, Genetic, Molecular Biology, Gene, Genetics, Regulation of gene expression, Uncoupling Agents, Membrane Transport Proteins, Proteins, Promoter, Cell Biology, Molecular biology, Gene Expression Regulation, chemistry, Protein Biosynthesis, Carrier Proteins, DNA
Abstract

Human and mouse UCP2 genes were cloned and sequenced. Transcriptional start sites were identified using primer extension analysis. The transcription unit of UCP2 gene is made of 2 untranslated exons followed by 6 exons encoding UCP2. In vitro translation analysis demonstrated that an open-reading-frame for a putative peptide of 36 residues present in exon 2 did not prevent UCP2 translation and confirmed that the initiation site of translation was in exon 3 as predicted from sequencing data. Short (bp -125 to +93) and long (bp -1383 and +93) CAT-constructs containing DNA upstream of the transcriptional start site of the human gene were made and transfected in adipocytes or HeLa cells allowing characterization of a potent promoter. Analysis of several genomic clones encompassing UCP2 and/or UCP3 genes demonstrated that the 2 genes are adjacent, the human UCP2 gene being located 7 kb downstream of the UCP3 gene.

Published
1999

2. Expression of the brown fat mitochondria uncoupling protein in Xenopus oocytes and import into mitochondrial membrane

Author

Susanne Klaus, Daniel Ricquier, Serge Raimbault, Louis Casteilla, and Frédéric Bouillaud

Subjects
Time Factors, Transcription, Genetic, Submitochondrial Particles, Biophysics, Xenopus, Mitochondrion, Biology, Biochemistry, Ion Channels, Mitochondrial Proteins, Xenopus laevis, Adipose Tissue, Brown, Salientia, Brown adipose tissue, medicine, Animals, Uncoupling protein, RNA, Messenger, Cloning, Molecular, Inner mitochondrial membrane, Molecular Biology, Uncoupling Protein 1, Messenger RNA, Membrane Proteins, Intracellular Membranes, Cell Biology, biology.organism_classification, Mitochondria, Kinetics, medicine.anatomical_structure, Membrane protein, Protein Biosynthesis, Oocytes, Female, Carrier Proteins, Plasmids
Abstract

Non shivering thermogenesis of brown adipose tissue is due to the uncoupling protein (UCP), located in the inner mitochondrial membrane, which functions as a proton translocator and can thus uncouple mitochondrial respiration. We describe here the expression of UCP in Xenopus laevis oocytes after injection of UCP mRNA, which was transcribed in vitro. UCP seems to be correctly transported into mitochondria and integrated into the membrane, but we were not able to establish definitely the functionality of this UCP. We conclude that this expression system could be suitable for the study of the mitochondrial import mechanism but not for the examination of physiological properties of UCP.

Published
1990

3. The gene for rat uncoupling protein: Complete sequence, structure of primary transcript and evolutionary relationship between exons

Author

Frédéric Bouillaud, Daniel Ricquier, and Serge Raimbault

Subjects
Molecular Sequence Data, Biophysics, Biology, Biochemistry, Ion Channels, Homology (biology), Mitochondrial Proteins, Gene product, Exon, Sequence Homology, Nucleic Acid, Animals, Uncoupling protein, RNA, Messenger, Cloning, Molecular, Molecular Biology, Gene, Uncoupling Protein 1, Genetics, Intron, Nucleic acid sequence, Membrane Proteins, Rats, Inbred Strains, Exons, Cell Biology, Molecular biology, Rats, Genes, Tandem exon duplication, Carrier Proteins, Mitochondrial ADP, ATP Translocases
Abstract

The complete nucleotide sequence of rat Uncoupling Protein gene has been determined. 4.5 Kb of the 5'-flanking region have also been sequenced. The site of transcription start as well as 3'-end extremities were identified. Transcription unit spans 8.4 Kb and contains 6 exons and 5 introns. Uncoupling protein as well as related mitochondrial carriers such as ADP/ATP carrier and phosphate carrier has a triplicated structure and each repeat of Uncoupling Protein corresponds to 2 exons. Two gene duplications could explain the triplicated structure of UCP and the more recent event of duplication concerned exons III and V. Evidence for homology between Uncoupling Protein and Neurospora crassa ADP/ATP carrier at the gene level was also noticed.

Published
1988

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