Search

The status of glyoxalase-I was explored in exponentially growing and G1arrested temperature sensitive (ts) cell division cycle (cdc) mutants of Saccharomyces cerevisiae. It was observed that the specific activity of this enzyme was correlated with overall growth status. The activity was high in actively growing cells and was low in G1arrested cells. Specific activities of glyoxalase-I were also low in G1arrested prolonged stationary phase (PSP) cells of S. cerevisiaeand Candida albicans. The activity of glyoxalase-I recovered when G1arrested S. cerevisiae(ts) cells were allowed to regrow under permissive conditions. Results demonstrate that although glyoxalase-I activity is a good indicator of cell growth status, it is not involved in cell cycle regulation of this eukaryotic organism.