1. Sialic acid-dependent attachment of mucins from three mouse strains to Entamoeba histolytica
- Author
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William A. Petri, Yasuhiro Takegawa, Katherine S. Ralston, Shinjiro Hamano, Carol A. Gilchrist, Yasuro Shinohara, and Kentaro Kato
- Subjects
Male ,Colon ,Biophysics ,Sialidase ,Biochemistry ,Article ,Host-Parasite Interactions ,Microbiology ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,Entamoeba histolytica ,Species Specificity ,Inbred strain ,Polysaccharides ,parasitic diseases ,Animals ,Glycomics ,Molecular Biology ,Glycoproteins ,030304 developmental biology ,chemistry.chemical_classification ,Mice, Inbred BALB C ,0303 health sciences ,biology ,Cell Membrane ,030302 biochemistry & molecular biology ,Mucin ,Mucins ,Rectum ,Lectin ,Cell Biology ,biology.organism_classification ,N-Acetylneuraminic Acid ,Sialic acid ,3. Good health ,Mice, Inbred C57BL ,carbohydrates (lipids) ,O-glycan ,chemistry ,Mice, Inbred CBA ,biology.protein ,Glycoprotein ,N-Acetylneuraminic acid ,Protein Binding - Abstract
Mouse strain-specific differences in the carbohydrate composition of intestinal mucins were hypothesized to account for strain-dependent susceptibility to Entamoeba histolytica. To test this hypothesis, intestinal mucins from susceptible and resistant inbred strains of mice were analyzed for their O-glycan content and for their ability to inhibit amoebic adherence to (GalNAc)12-27-HSA neo-glycoproteins. The results showed that the colorectal mucin O-glycan of susceptible CBA mice was lower in sialic acid content than that of resistant C57BL/6 and BALB/c mice. Mucins from CBA mice were more potent inhibitors of E. histolytica adherence to neo-glycoproteins than were mucins from C57BL/6 or BALB/c mice. Consistent with the role of terminal Gal/GalNAc as a receptor for amoebic adherence, sialidase treatment of C57BL/6 and BALB/c colorectal mucins increased their ability to inhibit E. histolytica adherence to the neo-glycoproteins. These results provide evidence of mouse strain-specific differences in the sialic acids content of mucin O-glycans. These dissimilarities likely contribute to the differential susceptibility of the three mouse strains to E. histolytica infection., Biochemical and Biophysical Research Communications, 436(2), pp.252-258; 2013
- Published
- 2013