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Start Over Author "Bernard Wortman" Journal biochemical and biophysical research communications
1 results on '"Bernard Wortman"'

1. Resolution of lactic dehydrogenase in beef cornea epithelium on diethylaminoethyl cellulose

Author

A. Schneider and Bernard Wortman

Subjects
chemistry.chemical_classification, Chromatography, Resolution (mass spectrometry), Chemistry, Biophysics, Cell Biology, Lactic dehydrogenase, Biochemistry, chemistry.chemical_compound, medicine.anatomical_structure, Cornea, Diethylaminoethyl cellulose, medicine, Cornea epithelium, Nucleotide, Cellulose, Molecular Biology, DEAE-Cellulose
Abstract

Multiple forms of lactic dehydrogenase (LDH) in beef cornea epithelial extract were studied by use of diethylaminoethyl (DEAE) cellulose anion-exchange chromatography. After passage through DEAE cellulose anion-exchange column, two forms of diphosphopyridine nucleotide (DPNH) linked LDH were detected. Both forms used triphosphopyridine nucleotide for the reduction of pyruvate to lactate but at a much lower rate than DPNH.

Published
1960
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