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Start Over Author "Akiko Amano" Journal biochemical and biophysical research communications
3 results on '"Akiko Amano"'

1. Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30/GNL knockout mice

Author

Hiroshi Obayashi, Akiko Amano, Toru Sasaki, Michiaki Fukui, Setsuko Handa, Goji Hasegawa, Toyotaka Mori, Yasunori Sato, Yoshitaka Kondo, Akihito Ishigami, Ryoya Takahashi, Mitsuhiro Ohta, Naoto Nakamura, Hikohito Fujinawa, Shizuo Kajiyama, and Naoki Maruyama

Subjects
Senescence, medicine.medical_specialty, Biophysics, Ascorbic Acid, medicine.disease_cause, Models, Biological, Biochemistry, Antioxidants, Mice, chemistry.chemical_compound, Superoxides, Internal medicine, medicine, Animals, Lucigenin, Molecular Biology, Mice, Knockout, Vitamin C, Superoxide, Body Weight, Calcium-Binding Proteins, Intracellular Signaling Peptides and Proteins, Brain, Water, Cell Biology, Ascorbic acid, Oxidative Stress, Endocrinology, chemistry, Knockout mouse, Gluconolactonase, Carboxylic Ester Hydrolases, Oxidative stress, Hydrogen
Abstract

Hydrogen is an established anti-oxidant that prevents acute oxidative stress. To clarify the mechanism of hydrogen's effect in the brain, we administered hydrogen-rich pure water (H(2)) to senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize vitamin C (VC), also a well-known anti-oxidant. These KO mice were divided into three groups; recipients of H(2), VC, or pure water (H(2)O), administered for 33 days. VC levels in H(2) and H(2)O groups were6% of those in the VC group. Subsequently, superoxide formation during hypoxia-reoxygenation treatment of brain slices from these groups was estimated by a real-time biography imaging system, which models living brain tissues, with Lucigenin used as chemiluminescence probe for superoxide. A significant 27.2% less superoxide formed in the H(2) group subjected to ischemia-reperfusion than in the H(2)O group. Thus hydrogen-rich pure water acts as an anti-oxidant in the brain slices and prevents superoxide formation.

Published
2008
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2. Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice

Author

Yoshitaka Kondo, Yasunori Sato, Akihito Ishigami, Kyu-Shik Jeong, Toru Sasaki, Setsuko Handa, Shingo Aizawa, Mizuki Iwama, Naoki Maruyama, Mitsugu Fukuda, Nobuko Shimada, Jaewon Lee, Masumi Akita, and Akiko Amano

Subjects
Antioxidant, medicine.medical_treatment, Biophysics, Ascorbic Acid, medicine.disease_cause, Biochemistry, Antioxidants, chemistry.chemical_compound, Mice, In vivo, Superoxides, medicine, Animals, Lucigenin, Molecular Biology, Mice, Knockout, Luminescent Agents, Superoxide, Body Weight, Calcium-Binding Proteins, Intracellular Signaling Peptides and Proteins, Brain, Cell Biology, Ascorbic acid, Molecular biology, chemistry, Knockout mouse, Gluconolactonase, Acridines, Carboxylic Ester Hydrolases, Oxidative stress
Abstract

Vitamin C (VC) has a strong antioxidant function evident as its ability to scavenge superoxide radicals in vitro. We verified that this property actually exists in vivo by using a real-time imaging system in which Lucigenin is the chemiluminescent probe for detecting superoxide in senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize VC in vivo. SMP30/GNL KO mice were given 1.5 g/L VC [VC(+)] for 2, 4, or 8 weeks or denied VC [VC(-)]. At 4 and 8 weeks, VC levels in brains from VC(-) KO mice were

Published
2008

3. Differences in the betaC-S lyase activities of viridans group streptococci

Author

Yasuo Yoshida, Masahiro Negishi, Takahiko Oho, Yoshio Nakano, and Akiko Amano

Subjects
DNA, Bacterial, animal structures, Biophysics, Biochemistry, Microbiology, Bacterial genetics, chemistry.chemical_compound, Cystathionine, Species Specificity, Humans, Cysteine, Hydrogen Sulfide, Molecular Biology, Cysteine metabolism, chemistry.chemical_classification, biology, Base Sequence, Molecular Structure, Cell Biology, Lyase, Viridans Streptococci, Cystathionine beta synthase, Recombinant Proteins, Amino acid, Carbon-Sulfur Lyases, Enzyme, chemistry, Genes, Bacterial, Streptococcus anginosus, biology.protein
Abstract

betaC-S Lyase catalyzes the alpha,beta-elimination of L-cysteine to hydrogen sulfide, which is one of the main causes of oral malodor and is highly toxic to mammalian cells. We evaluated the capacity of six species of oral streptococci to produce hydrogen sulfide. The crude enzyme extract from Streptococcus anginosus had the greatest capacity. However, comparative analysis of amino acid sequences did not detect any meaningful differences in the S. anginosus betaC-S lyase. The capacity of S. anginosus purified betaC-S lyase to degrade L-cysteine was also extremely high, while its capacity to degrade L-cystathionine was unremarkable. These findings suggest that the extremely high capacity of S. anginosus to produce hydrogen sulfide is due to the unique characteristic of betaC-S lyase from that organism.

Published
2002

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