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Curcuma longa, often known as turmeric, is a highly regarded and extensively utilised medicinal plant native to India. It belongs to the Zingiberaceae family. Curcumin, Demethoxycurcumin (DMC) and Bisdemethoxycurcumin (BDMC) are the chemical components found in turmeric and are together referred to as curcuminoids. Curcumin, also known as Diferuloylmethane, is a compound that is widely recognised for its diverse range of biological effects. This document presents a discussion on several clinical trials and their corresponding observations related to these activities. Curcumin is a chemical that exists in two different forms depending on the solvent it is in. In organic solvents, it exists in the enolic form, while in water, it exists in the keto form. This review article provides a comprehensive overview of the several functions and activities of Curcumin. [ABSTRACT FROM AUTHOR]

Turmeric has traditionally been employed as a potent anti-inflammatory agent in Indian medicinal practices. Turmeric has historically been referred to as "Indian saffron" due to its vibrant yellow-orange hue and it has been utilised as a seasoning, medicinal treatment, and fabric dye. Turmeric contains a high concentration of curcuminoids, which have diverse chemical structures and physico-chemical properties. Distinctive attributes or qualities. This study presents a method for extracting curcuminoids utilizing a Soxhlet extractor machine. The process of purifying and measuring the amount of curcuminoids was conducted using thin layer chromatography. The maximal resulting extract was obtained by the process of TLC using Hexane as the solvent. The Soxhlet extraction procedure yielded a curcumin percentage of 4.09%. Various solvents were employed for the extraction process, and it was found that acetone exhibited the highest yield of each curcuminoid. The separation of curcuminoids was examined using thin-layer chromatography (TLC) with a chloroform: methanol solvent mixture at a ratio of 95:5. The resulting RF values for curcumin, di-methoxy-curcumin and bis de-methoxy-curcumin were 0.59, 0.61 and 0.58, respectively. [ABSTRACT FROM AUTHOR]

Breast cancer is a complex illness with many different types. In the Western world, it is the largest cause of cancer-related death among women. With the rising prevalence of breast cancer, particularly lymph node negative breast cancer, precise prediction is required to ensure successful treatments and avoid under or overtreatment. Various prognostic indicators have been created in an attempt to provide reliable diagnosis and determine which therapy is best for a certain patient. Mitochondrial mito-stress testing was utilized to look at basic cell metabolism, such as ATP turnover, protons leak, maximum respiration, and reserve respiration capabilities. All of these measures were found to be lower in MCF-7 cells transfected with let-7b inhibitors than in control cells transfected with scramble, resulting in a significant reduction in ATP generation. [ABSTRACT FROM AUTHOR]

The people can survive day, weak and month with eat food, but without water people cannot survive for a day. This contamination of drinking water due to (E coli, Salmonella, Shigella, and cholera), viruses (Hepatitis A, Polio Virus and Rota Virus). In this study, drinking water collected from different area of Allahabad District. We performed the microbiological, biochemical, Molecular biological, Molecular biological to confirm the presence of E. coli, Salmonella and Shigella etc. type of pathogenic bacteria in different area of drinking water. Molecular based method is evaluated by the 16S r DNA gene sequencing technique to characterized the pathogenic bacteria belonging to family Enterobacteriaceae. Design and synthesis a set of oligonucleotides (Primer) capable of amplifying nearly full length (1500bp) 16S r-DNA from many bacteria like Clastridium perfringer, pseudomonas, Neissiria, Gonorrhaeae, Proteus, Escherichia, Eracinia, antibiotics such as Ampicillin, streptomycin, Gentamycin Ciprofloxacine and observed high background of resistant and virulence bacteria in Tap water. [ABSTRACT FROM AUTHOR]

Influenza viruses have acquired resistance to approved neuraminidase-targeting drugs, increasing the need for new drug targets for the development of novel anti-influenza drugs. The control of influenza virus infection is a major public health concern due to the significant morbidity and mortality it causes through seasonal epidemics and pandemics. I try to find those drugs which are based on Lipinski rule for this. we used data for the collect information in the study and abstracted the following databases, which is PubChem and drug bank. I retrieved 20 drug of swine flu from drug bank and retrieve the similar drug from Pubchem database and perform clustering of drug to group than on the basis of chemical properties and further observe that which drugs are follow the Lipinski rule which is based on in-silico method. Further, the selected drug structure was docked with chemical compounds using AutoDock1.5.6. This study also highlights stronger binding energy having good ADMET property with H1N1 drugs. [ABSTRACT FROM AUTHOR]

Breast cancer is a complex illness with many different types. In the Western world, it is the largest cause of cancer-related death among women. Utilizing quantitative real PCR, the expression levels of nine distinct miRNAs (let-7b, miR-18b, miR-21, miR-25, miR-29c, miR-106b, miR375, miR505 and miR-150) were studied (qPCR). To isolate total RNA, researchers used 123 formaldehyde fixed paraffin embedded (FFPE) tissues. TILs (tumor infiltrating lymphocytes) were examined in the samples to see if they had any predictive value. Let-7b's biological activity in ER negative (MDAMB-231) and positive (MCF-7) breast cancer cell lines was investigated among the miRNAs tested. PNATM inhibitor was delivered for this purpose, and the associated miRNA was evaluated using qPCR. A wound healing experiment was used to investigate the transfected cells' rate of proliferation and migratory potential. The presence of markers in the let-7b inhibited was examined using immunohistochemistry. [ABSTRACT FROM AUTHOR]