1. Fluorescence probing of T box antiterminator RNA: Insights into riboswitch discernment of the tRNA discriminator base
- Author
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Means, John A., Simson, Crystal M., Zhou, Shu, Rachford, Aaron A., Rack, Jeffrey J., and Hines, Jennifer V.
- Subjects
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TRANSFER RNA , *FLUORESCENCE , *GRAM-positive bacteria , *AMINO acid metabolism , *GENETIC regulation , *ADENINE , *FLUORESCENT probes , *GENETIC models - Abstract
Abstract: The T box transcription antitermination riboswitch is one of the main regulatory mechanisms utilized by Gram-positive bacteria to regulate genes that are involved in amino acid metabolism. The details of the antitermination event, including the role that Mg2+ plays, in this riboswitch have not been completely elucidated. In these studies, details of the antitermination event were investigated utilizing 2-aminopurine to monitor structural changes of a model antiterminator RNA when it was bound to model tRNA. Based on the results of these fluorescence studies, the model tRNA binds the model antiterminator RNA via an induced-fit. This binding is enhanced by the presence of Mg2+, facilitating the complete base pairing of the model tRNA acceptor end with the complementary bases in the model antiterminator bulge. [Copyright &y& Elsevier]
- Published
- 2009
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