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Microorganisms can be photoinactivated with 405 and 450 nm irradiation, due to endogenous photosensitizers, which absorb light of these wavelengths and generate reactive oxygen species that destroy the cells from within. The photosensitizers assumed to be responsible are porphyrins in the spectral region around 405 nm and flavins at about 450 nm. The aim of this study was to investigate this hypothesis on enterococci, considering that they do not contain porphyrins. In photoinactivation experiments with Enterococcus moraviensis, 405 nm and 450 nm irradiation both led to a reduction of the bacterial concentration by several orders of magnitude with 405 nm irradiation being much more efficient. The measurement and analysis of the fluorescence spectra revealed no signs of porphyrins whereas flavins seemed to be rapidly converted to lumichrome by 405 nm radiation. Therefore, probably none of the usual suspects, porphyrins and flavins, was responsible for the photoinactivation of Enterococcus moraviensis during 405 nm irradiation. Fluorescence experiments revealed the spectra of lumichrome and NADH, which are both known photosensitizers. Presumably, one of them or both were actually involved here. As NADH and flavins (and therefore their photodegradation product lumichrome) are abundant in all microorganisms, they are probably also involved in 405 nm photoinactivation processes of other species. • Violet (405 nm) and blue (450 nm) light photoinactivates enterococci. • Neither porphyrins nor flavins seem to be involved in this photoinactivation. • The photosensitizer lumichrome is generated by 405 nm irradiation. • Lumichrome or NADH are probably the responsible photosensitizers for 405 nm photoinactivation. [ABSTRACT FROM AUTHOR]