1. One-step construction of a molybdenum disulfide/multi-walled carbon nanotubes/polypyrrole nanocomposite biosensor for the ex-vivo detection of dopamine in mouse brain tissue.
- Author
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Vijayaraj, Kathiresan, Dinakaran, Thirumalai, Lee, Yujeong, Kim, Suhkmann, Kim, Hyung Sik, Lee, Jaewon, and Chang, Seung-Cheol
- Subjects
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MOLYBDENUM disulfide , *MULTIWALLED carbon nanotubes , *NANOCOMPOSITE materials , *BIOSENSORS , *BRAIN physiology , *PHYSIOLOGICAL effects of dopamine - Abstract
We developed a new strategy for construction of a biosensor for the neurotransmitter dopamine. The biosensor was constructed by one-step electrochemical deposition of a nanocomposite in aqueous solution at pH 7.0, consisting of molybdenum disulfide, multi-walled carbon nanotubes, and polypyrrole. A series of analytical methods was performed to investigate the surface characteristics and the improved electrocatalytic effect of the nanocomposite, including cyclic voltammetry, electrochemical impedance spectroscopy, field-emission scanning electron microscopy, atomic force microscopy, and Raman spectroscopy. The constructed biosensor showed high sensitivity (1.130 μAμM −1 cm −2 ) with a dynamic linearity range of 25–1000 nM and a detection limit of 10 nM. Additionally, the designed sensor exhibited strong anti-interference ability and satisfactory reproducibility. The practical application of the sensor was manifested for the ex vivo determination of dopamine neurotransmitters using brain tissue samples of a mouse Parkinson's disease model. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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