1. Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice
- Author
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Kondo, Yoshitaka, Sasaki, Toru, Sato, Yasunori, Amano, Akiko, Aizawa, Shingo, Iwama, Mizuki, Handa, Setsuko, Shimada, Nobuko, Fukuda, Mitsugu, Akita, Masumi, Lee, Jaewon, Jeong, Kyu-Shik, Maruyama, Naoki, and Ishigami, Akihito
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VITAMIN C , *SUPEROXIDES , *ANTIOXIDANTS , *LUMINESCENT probes , *LABORATORY mice , *REACTIVE oxygen species , *RADICALS (Chemistry) - Abstract
Abstract: Vitamin C (VC) has a strong antioxidant function evident as its ability to scavenge superoxide radicals in vitro. We verified that this property actually exists in vivo by using a real-time imaging system in which Lucigenin is the chemiluminescent probe for detecting superoxide in senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize VC in vivo. SMP30/GNL KO mice were given 1.5g/L VC [VC(+)] for 2, 4, or 8 weeks or denied VC [VC(−)]. At 4 and 8 weeks, VC levels in brains from VC(−) KO mice were <6% of that in VC(+) KO mice. Accordingly, superoxide-dependent chemiluminescence levels determined by ischemia-reperfusion at the 4- and 8 weeks test intervals were 3.0-fold and 2.1-fold higher, respectively, in VC(−) KO mice than in VC(+) KO mice. However, total superoxide dismutase activity and protein levels were not altered. Thus, VC depletion specifically increased superoxide generation in a model of the living brain. [Copyright &y& Elsevier]
- Published
- 2008
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