1. The tetrapeptide Arg-Leu-Tyr-Glu inhibits VEGF-induced angiogenesis.
- Author
-
Baek, Yi-Yong, Lee, Dong-Keon, So, Ju-Hoon, Kim, Cheol-Hee, Jeoung, Dooil, Lee, Hansoo, Choe, Jongseon, Won, Moo-Ho, Ha, Kwon-Soo, Kwon, Young-Guen, and Kim, Young-Myeong
- Subjects
- *
PEPTIDE analysis , *VASCULAR endothelial growth factors , *PLASMINOGEN , *NEOVASCULARIZATION , *ENDOTHELIAL cells , *CELL proliferation - Abstract
Kringle 5, derived from plasminogen, is highly capable of inhibiting angiogenesis. Here, we have designed and synthesized 10 tetrapeptides, based on the amino acid properties of the core tetrapeptide Lys-Leu-Tyr-Asp (KLYD) originating from anti-angiogenic kringle 5 of human plasminogen. Of these, Arg-Leu-Tyr-Glu (RLYE) effectively inhibited vascular endothelial growth factor (VEGF)-induced endothelial cell proliferation, migration and tube formation, with an IC 50 of 0.06–0.08 nM, which was about ten-fold lower than that of the control peptide KLYD (0.79 nM), as well as suppressed developmental angiogenesis in a zebrafish model. Furthermore, this peptide effectively inhibited the cellular events that precede angiogenesis, such as ERK and eNOS phosphorylation and nitric oxide production, in endothelial cells stimulated with VEGF. Collectively, these data demonstrate that RLYE is a potent anti-angiogenic peptide that targets the VEGF signaling pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF