1. Novel automated immune turbidimetric assay for routine urinary cystatin-C determinations
- Author
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Ágnes Lakatos, Andrea Ludány, Balázs Szirmay, István Wittmann, Diána Mühl, Tamás Kőszegi, Zoltán Horváth-Szalai, Péter Kustán, Attila Miseta, and Gábor L. Kovács
- Subjects
030213 general clinical medicine ,medicine.medical_specialty ,Urinary system ,Clinical Biochemistry ,Urology ,Urine ,030204 cardiovascular system & hematology ,Analytical Chemistry ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Nephelometry and Turbidimetry ,Medicine ,Humans ,General Pharmacology, Toxicology and Pharmaceutics ,Cystatin C ,biology ,business.industry ,Acute kidney injury ,General Medicine ,medicine.disease ,Medical Laboratory Technology ,biology.protein ,Turbidimetry ,business - Abstract
Aim: There is no commercially available urinary cystatin-C (u-CYSC) test in the market. Therefore, we optimized and validated an automated immune turbidimetric test for u-CYSC measurements and investigated u-CYSC concentrations in acute and chronic diseases which might lead to renal tubular disorders. Materials & methods: A particle-enhanced immune turbidimetric assay was adapted and validated on a Cobas 8000/c502 analyzer. Urine samples of different patient groups were also analyzed. Results: Our method showed excellent analytical performance. U-CYSC/u-creatinine (u-CREAT) was higher in sepsis-related acute kidney injury group (p
- Published
- 2018