1. Analytical comparison of ELISA and mass spectrometry for quantification of serum hepcidin in critically ill patients
- Author
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Constance Delaby, Jérôme Vialaret, Christophe Hirtz, Thibaud Lefebvre, Matthias Herkert, Hervé Puy, Sigismond Lasocki, Sylvain Lehmann, Pierre Asfar, Alain Mercat, Thomas Gaillard, Soizic Gergaud, Cyrille Sargentini, Claire Geneve, Philippe Montravers, Thibault Lefebvre, Herve Puy, Grégoire Mercier, Nicolas Nagot, Gerald Chanques, Samir Jaber, Karim Asehnoune, Antoine Roquilly, Claire Dahyot-Fizelier, Olivier Mimoz, Sonia Isslame, Philippe Seguin, Mathilde Barbaz, Martine Ferrandiere, Thomas Kerforne, Katell Peoc'h, François Beloncle, Maxime Leger, Emmanuel Rineau, Gregory Marin, Matthieu Boisson, Yoann Launey, Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)
- Subjects
030213 general clinical medicine ,medicine.medical_specialty ,Anemia ,Critical Illness ,[SDV]Life Sciences [q-bio] ,Clinical Biochemistry ,Enzyme-Linked Immunosorbent Assay ,Mass spectrometry ,Gastroenterology ,Mass Spectrometry ,Analytical Chemistry ,03 medical and health sciences ,0302 clinical medicine ,Hepcidins ,Hepcidin ,Internal medicine ,Humans ,Protein Isoforms ,Medicine ,General Pharmacology, Toxicology and Pharmaceutics ,Anemia, Iron-Deficiency ,biology ,business.industry ,Critically ill ,030208 emergency & critical care medicine ,General Medicine ,medicine.disease ,3. Good health ,Medical Laboratory Technology ,Iron-deficiency anemia ,biology.protein ,Reagent Kits, Diagnostic ,business - Abstract
International audience; Aim: To compare methods of quantifying serum hepcidin (based on MS and ELISA) and their ability to diagnose true iron deficiency anemia in critically ill patients. Materials & methods: Serum hepcidin was measured in 119 critically ill patients included in the HEPCIDANE clinical trial, using either an ultra-sensitive ELISA kit (from DRG) or two different MS methods. Results: The results show a good correlation between the different methods studied. The Bland–Altman analysis and the Kappa test for clinical groups show a good or very good agreement between the different tests. Conclusion: ELISA or MS show a satisfactory commutability to quantify serum hepcidin. This is of great importance for the determination of therapeutic strategies in iron deficiency.
- Published
- 2021
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