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2 results on '"Kelly H. Oh"'

1. Aldicarb-induced Paralysis Assay to Determine Defects in Synaptic Transmission in

Author

Kelly H, Oh and Hongkyun, Kim

Subjects
Article
Abstract

Aldicarb treatment causes an accumulation of acetylcholine in the synaptic cleft of the neuromuscular junction, resulting in sustained muscle activation and eventually paralysis. Aldicarb-induced paralysis assay is an easy and fast method to determine whether synaptic transmission of a C. elegans mutant of interest is altered. This assay is based on the correlation of the rate of neurotransmitter release with the rate of paralysis. In this protocol, we describe a method for simultaneously assessing the aldicarb sensitivity of animals with different genotypes.

Published
2017

2. Aldicarb-induced Paralysis Assay to Determine Defects in Synaptic Transmission in Caenorhabditis elegans

Author

Hongkyun Kim and Kelly H. Oh

Subjects
0301 basic medicine, Synaptic cleft, Strategy and Management, Neurotransmission, Industrial and Manufacturing Engineering, Neuromuscular junction, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Paralysis, medicine, Neurotransmitter, Caenorhabditis elegans, biology, Mechanical Engineering, Metals and Alloys, Anatomy, biology.organism_classification, Acetylcholinesterase, Cell biology, 030104 developmental biology, medicine.anatomical_structure, chemistry, medicine.symptom, 030217 neurology & neurosurgery, Acetylcholine, medicine.drug
Abstract

Aldicarb treatment causes an accumulation of acetylcholine in the synaptic cleft of the neuromuscular junction, resulting in sustained muscle activation and eventually paralysis. Aldicarb-induced paralysis assay is an easy and fast method to determine whether synaptic transmission of a C. elegans mutant of interest is altered. This assay is based on the correlation of the rate of neurotransmitter release with the rate of paralysis. In this protocol, we describe a method for simultaneously assessing the aldicarb sensitivity of animals with different genotypes.

Published
2017
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