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2 results on '"Beth Mann"'

1. Preparation of Purified Gram-positive Bacterial Cell Wall and Detection in Placenta and Fetal Tissues

Author

Beth Mann, Lip Loh, Geli Gao, and Elaine Tuomanen

Subjects
Biology (General), QH301-705.5
Abstract

Cell wall is a complex biopolymer on the surface of all Gram-positive bacteria. During infection, cell wall is recognized by the innate immune receptor Toll-like receptor 2 causing intense inflammation and tissue damage. In animal models, cell wall traffics from the blood stream to many organs in the body, including brain, heart, placenta and fetus. This protocol describes how to prepare purified cell wall from Streptococcus pneumoniae, detect its distribution in animal tissues, and study the tissue response using the placenta and fetal brain as examples.

Published
2016
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2. Preparation of Purified Gram-positive Bacterial Cell Wall and Detection in Placenta and Fetal Tissues

Author

Geli Gao, Beth Mann, Elaine Tuomanen, and Lip Nam Loh

Subjects
Fetus, Innate immune system, Strategy and Management, Mechanical Engineering, Metals and Alloys, Inflammation, Biology, Industrial and Manufacturing Engineering, Bacterial cell structure, Article, Cell biology, Cell wall, chemistry.chemical_compound, medicine.anatomical_structure, chemistry, Placenta, Immunology, embryonic structures, medicine, Peptidoglycan, medicine.symptom, Receptor
Abstract

Cell wall is a complex biopolymer on the surface of all Gram-positive bacteria. During infection, cell wall is recognized by the innate immune receptor Toll-like receptor 2 causing intense inflammation and tissue damage. In animal models, cell wall traffics from the blood stream to many organs in the body, including brain, heart, placenta and fetus. This protocol describes how to prepare purified cell wall from Streptococcus pneumoniae, detect its distribution in animal tissues, and study the tissue response using the placenta and fetal brain as examples.

Published
2017

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