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The operating room is an important place for the treatment of patients undergoing surgery, the quality level of clinical nursing has a direct influence in the therapeutic effect of patients. In the present study, four components instructional design mode (4C training mode) was applied in the training of professional nurses in the operating room, it involved the following aspects: regular skill knowledge analysis, goals setting, as well as the construction and implementation of the project. Targeted training methods and training content were formulated in accordance with the learning ability and knowledge reserve of different professional nurses, and different skills required to master, to fully mobilize the enthusiasm and initiative of nurses, thereby to improve the operation and comprehensive level of nurses in the operation room to achieve good results.