1. [Comparison of characteristics of SVCV strains isolated in China and in Europe].
- Author
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Gao LY, Liu H, Shi XJ, He JQ, and Jiang YL
- Subjects
- Animals, Cell Line, China, Europe, Fishes, Microscopy, Electron, Transmission, Reverse Transcriptase Polymerase Chain Reaction, Rhabdoviridae Infections virology, Vesiculovirus growth & development, Vesiculovirus isolation & purification, Vesiculovirus ultrastructure, Carps virology, Fish Diseases virology, Rhabdoviridae Infections veterinary, Vesiculovirus genetics
- Abstract
In the nationwide epidemiological investigation, SVCV-741 was for the first time isolated in Beijing region, China in 2003, and designated as SVCV Asian strain. In this paper, we compared SVCV-741 (Asian strains isolated in China) with SVCV-10/3 (Europe reference strain) on their physico-chemical, biological and morphological characteristics. The results indicated that there were no distinct differences between two SVCV strains on phycico-chemical and morphological characteristics. The main existing differences were: (1) The stability of SVCV-741 to temperature in cell culture was higher than that of SVCV-10/3, which might have some evolutionary and biological implication of SVCV; (2) No SVC outbreak ever occurred caused by SVCV-741;Furthermore we found that both SVCV-741 and SVCV-10/3 grew faster and produced higher virus titer in CO cells than other cell lines. It indicated that CO cell lines might be useful tool for SVCV research.
- Published
- 2009