1. Antidepressant-like effect of fluoxetine may depend on translocator protein activity and pretest session duration in forced swimming test in mice.
- Author
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Kudryashov NV, Kalinina TS, Shimshirt AA, Korolev AO, Volkova AV, and Voronina TA
- Subjects
- Animals, Antidepressive Agents pharmacology, Depression drug therapy, Depressive Disorder drug therapy, Disease Models, Animal, Isoquinolines pharmacology, Male, Mice, Mice, Inbred ICR, Mitochondrial Trifunctional Protein metabolism, Mitochondrial Trifunctional Protein physiology, Motor Activity drug effects, Selective Serotonin Reuptake Inhibitors pharmacology, Signal Transduction drug effects, Stress, Psychological drug therapy, Swimming, Fluoxetine metabolism, Fluoxetine pharmacology, Mitochondrial Trifunctional Protein drug effects
- Abstract
The antidepressant-like effect of fluoxetine (20 mg/kg i.p.) has been assessed using the forced swimming test (FST) in IRC (CD-1) mice exposed or not to a pretest session of different duration (5 or 20 min). The influence of the mitochondrial translocator protein (TSPO) activity on the antidepressant-like effect of fluoxetine (20 mg/kg i.p.) in the FST was also studied. The antidepressant-like effect of fluoxetine was observed only in mice subjected to a 5-min pretest session 24 h before the FST. The TSPO antagonist PK11195 [1-(2-chlorophenyl)-N-methyl-N-(1-methylpropyl)-3-isoquinolinecarboxamide; 1 or 3 mg/kg i.p.] inhibited the antidepressant activity of fluoxetine in the FST. In the present study, fluoxetine or PK11195 was administered for a short duration. We suppose that the functional activity of TSPO may depend on a pretest session and that using this procedure is necessary to detect antidepressant activity of fluoxetine-like drugs.
- Published
- 2018
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