1. Evidence for Association Between Low Frequency Variants in CHRNA6/CHRNB3 and Antisocial Drug Dependence
- Author
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Kamens, Helen M, Corley, Robin P, Richmond, Phillip A, Darlington, Todd M, Dowell, Robin, Hopfer, Christian J, Stallings, Michael C, Hewitt, John K, Brown, Sandra A, and Ehringer, Marissa A
- Subjects
Pharmacology and Pharmaceutical Sciences ,Biomedical and Clinical Sciences ,Genetics ,Brain Disorders ,Clinical Research ,2.1 Biological and endogenous factors ,Aetiology ,Good Health and Well Being ,Adolescent ,Antisocial Personality Disorder ,Female ,Gene Frequency ,Genetic Association Studies ,Genetic Predisposition to Disease ,Humans ,Male ,Quality Control ,Receptors ,Nicotinic ,Software ,Young Adult ,Low frequency variants ,Antisocial drug dependence ,Association ,Sequence ,Nicotinic acetylcholine receptor ,Zoology ,Neurosciences ,Psychology ,Genetics & Heredity ,Biomedical and clinical sciences ,Health sciences - Abstract
Common SNPs in nicotinic acetylcholine receptor genes (CHRN genes) have been associated with drug behaviors and personality traits, but the influence of rare genetic variants is not well characterized. The goal of this project was to identify novel rare variants in CHRN genes in the Center for Antisocial Drug Dependence (CADD) and Genetics of Antisocial Drug Dependence (GADD) samples and to determine if low frequency variants are associated with antisocial drug dependence. Two samples of 114 and 200 individuals were selected using a case/control design including the tails of the phenotypic distribution of antisocial drug dependence. The capture, sequencing, and analysis of all variants in 16 CHRN genes (CHRNA1-7, 9, 10, CHRNB1-4, CHRND, CHRNG, CHRNE) were performed independently for each subject in each sample. Sequencing reads were aligned to the human reference sequence using BWA prior to variant calling with the Genome Analysis ToolKit (GATK). Low frequency variants (minor allele frequency
- Published
- 2016