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This paper presents the discrete version of the Mycorrhiza Tree Optimization Algorithm (MTOA), using the Lotka–Volterra Discrete Equation System (LVDES) formed by the Predator–Prey, Cooperative and Competitive Models. The Discrete Mycorrhizal Optimization Algorithm (DMOA) is a stochastic metaheuristic that integrates randomness in its search processes. These algorithms are inspired by nature, specifically by the symbiosis between plant roots and a fungal network called the Mycorrhizal Network (MN). The communication in the network is performed using chemical signals of environmental conditions and hazards, the exchange of resources, such as Carbon Dioxide (CO2) that plants perform through photosynthesis to the MN and to other seedlings or growing plants. The MN provides water (H2O) and nutrients to plants that may or may not be of the same species; therefore, the colonization of plants in arid lands would not have been possible without the MN. In this work, we performed a comparison with the CEC-2013 mathematical functions between MTOA and DMOA by conducting Hypothesis Tests to obtain the efficiency and performance of the algorithms, but in future research we will also propose optimization experiments in Neural Networks and Fuzzy Systems to verify with which methods these algorithms perform better. [ABSTRACT FROM AUTHOR]