1. Fluoroquinolone and macrolide resistance inCampylobacter jejuniisolated from broiler slaughterhouses in southern Brazil
- Author
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Gustavo Perdoncini, Yuli M. Sierra-Arguello, Vladimir Pinheiro do Nascimento, Marcos José Pereira Gomes, Carlos Tadeu Pippi Salle, R. B. Morgan, and H. L. S. Moraes
- Subjects
0301 basic medicine ,Bacterial Gastroenteritis ,Nalidixic acid ,030106 microbiology ,Population ,Erythromycin ,Microbial Sensitivity Tests ,Campylobacter jejuni ,Microbiology ,Nalidixic Acid ,03 medical and health sciences ,Antibiotic resistance ,Food Animals ,Ciprofloxacin ,Drug Resistance, Multiple, Bacterial ,Campylobacter Infections ,medicine ,Animals ,Humans ,education ,Poultry Diseases ,education.field_of_study ,General Immunology and Microbiology ,biology ,biology.organism_classification ,Antimicrobial ,Anti-Bacterial Agents ,030104 developmental biology ,Animal Science and Zoology ,Macrolides ,Chickens ,Abattoirs ,Brazil ,Polymorphism, Restriction Fragment Length ,Fluoroquinolones ,medicine.drug - Abstract
Campylobacter jejuni is recognized as a leading cause of acute bacterial gastroenteritis in humans. The over-use of antimicrobials in the human population and in animal husbandry has led to an increase in antimicrobial-resistant infections, particularly with fluoroquinolones and macrolides. The aim of the present study was to provide information of the current status of antimicrobial resistance patterns in Campylobacter jejuni from poultry sources. Fifty strains were recovered from broiler slaughterhouses in Rio Grande do Sul state, Brazil, 2012. The strains were investigated for antimicrobial susceptibility against three agents (ciprofloxacin, nalidixic acid and erythromycin) by minimal inhibitory concentrations. The strains were analysed by polymerase chain reaction-restriction fragment length polymorphism for detection of the Thr-86 mutation that confers resistance to ciprofloxacin. In addition, all the strains were tested for the presence of efflux systems (cmeB gene) conferring antimicrobial resistance. The minimum inhibitory concentrations results showed that 98% of isolates were sensitive to erythromycin and most isolates were resistant to ciprofloxacin (94%) and nalidixic acid (90%). A complete correlation was observed between the minimum inhibitory concentrations and PCR-RFLP assay. Finally, the cmeB gene that is responsible for multidrug resistance was detected in 16 isolates out the 50 strains (32%).
- Published
- 2016