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Start Over Descriptor "EBSS, Earle's balanced salt solution" Journal autophagy
8 results on '"EBSS, Earle's balanced salt solution"'

8. The integral membrane protein ITM2A, a transcriptional target of PKA-CREB, regulates autophagic flux via interaction with the vacuolar ATPase

Author

Ik Soon Jang, Sim Namkoong, Kang I. Lee, Rackhyun Park, Junsoo Park, Eun-Ju Lee, and Jin I. Lee

Subjects
tfLC3, tandem fluorescent-tagged LC3, v-ATPase, vacuolar ATPase, Transcription, Genetic, Phagosomes, LAMP1, lysosomal-associated membrane protein 1, PKA, SQSTM1, sequestosome 1, Cyclic AMP Response Element-Binding Protein, Promoter Regions, Genetic, Integral membrane protein, biology, CREB, BafA1, bafilomycin A1, Basic Research Paper, Cell biology, cAMP, cyclic adenosine monophosphate, ChIP, chromatin immunoprecipitation, v-ATPase, CRE, cAMP response element, EBSS, Earle's balanced salt solution, Signal transduction, Protein Binding, Signal Transduction, autophagy, Vacuolar Proton-Translocating ATPases, CREB, cAMP responsive element binding protein, MTOR, mechanistic target of rapamycin, ITM2A, integral membrane protein 2A, TPA, 12-O-tetradecanoylphorbol-13-acetate, Models, Biological, V-ATPase, Humans, Gene Silencing, Molecular Biology, MAP1LC3B/LC3B, microtubule-associated protein 1 light chain 3 β, Autophagy, Membrane Proteins, Cell Biology, Cyclic AMP-Dependent Protein Kinases, ITM2A, HEK293 Cells, Membrane protein, biology.protein, PKA, protein kinase A, Lysosomes, Flux (metabolism), MAPK, mitogen-activated protein kinase, HeLa Cells
Abstract

The PKA-CREB signaling pathway is involved in many cellular processes including autophagy. Recent studies demonstrated that PKA-CREB inhibits autophagy in yeast; however, the role of PKA-CREB signaling in mammalian cell autophagy has not been fully characterized. Here, we report that the integral membrane protein ITM2A expression is positively regulated by PKA-CREB signaling and ITM2A expression interferes with autophagic flux by interacting with vacuolar ATPase (v-ATPase). The ITM2A promoter contains a CRE element, and mutation at the CRE consensus site decreases the promoter activity. Forskolin treatment and PKA expression activate the ITM2A promoter confirming that ITM2A expression is dependent on the PKA-CREB pathway. ITM2A expression results in the accumulation of autophagosomes and interferes with autolysosome formation by blocking autophagic flux. We demonstrated that ITM2A physically interacts with v-ATPase and inhibits lysosomal function. These results support the notion that PKA-CREB signaling pathway regulates ITM2A expression, which negatively regulates autophagic flux by interfering with the function of v-ATPase.

Published
2015

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