1. Passive Transfer of Adjuvant-Induced Arthritis Into Irradiated Da Recipient Rats
- Author
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Frederic Clayton, D. Scott Harper, Grant W. Cannon, and Marie M. Griffiths
- Subjects
Male ,Pathology ,medicine.medical_specialty ,Cell Transplantation ,medicine.medical_treatment ,Freund's Adjuvant ,Immunology ,Arthritis ,Spleen ,Pharmacology ,Immunotherapy, Adoptive ,medicine ,Splenocyte ,Animals ,Immunology and Allergy ,Lymph node ,biology ,business.industry ,Rats, Inbred Strains ,medicine.disease ,Arthritis, Experimental ,In vitro ,Rats ,medicine.anatomical_structure ,Concanavalin A ,Cell culture ,biology.protein ,business ,Adjuvant ,Whole-Body Irradiation - Abstract
Adjuvant-induced arthritis (AIA) can be passively transferred in Dark Agouti (DA) rats by spleen and lymph node cells after culture with Concanavalin A (Con A). A model not requiring in vitro Con A expansion and activation would be important in investigations of anti-rheumatic drugs in AIA. A new model using irradiated recipients fills this need. Donor DA rats treated with 0.1 ml complete Freund's adjuvant (CFA) containing 7.5 mg M. butyricum/ml were sacrificed 11 days after CFA injection, donor spleen cells harvested, and donor spleen cells injected intravenously into recipient DA rats previously irradiated with 5 Gy. Recipient rats developed arthritis 4-14 days after spleen cell transfer. This model can now be used to further define the effects of anti-rheumatic drugs in the passive transfer of AIA by eliminating the need for the in vitro Con A-induced expansion and/or activation of donor cells.
- Published
- 1993
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