1. Analysis of pancreas-infiltrating T cells in diabetic NOD mice: fusion with BW5147 yields a high frequency of islet-reactive hybridomas.
- Author
-
Prud'homme GJ, Long TY, Bocarro DC, Balderas RS, and Theofilopoulos AN
- Subjects
- Animals, Animals, Newborn, Antigens, Differentiation, T-Lymphocyte immunology, CD3 Complex, CD4 Antigens immunology, CD8 Antigens immunology, Diabetes Mellitus, Type 1 immunology, Hybridomas metabolism, Interleukin-2 metabolism, Islets of Langerhans immunology, Mice, Mice, Inbred NOD, Receptors, Antigen, T-Cell immunology, Hybridomas immunology, Pancreas immunology, T-Lymphocytes immunology
- Abstract
Diabetes in NOD mice is an autoimmune disease which is characterized by the infiltration of islets of Langerhans by large numbers of T cells. Some of these infiltrating T cells are clearly islet-cells-specific; however, many or most of these T cells could be attracted nonspecificity into these lesions. To study NOD pancreas-infiltrating T cells, we fused these cells with BW5147 to make T cell hybridomas. Ninety-four pancreas-derived T hybrids were analyzed of which 12 responded specifically to islet cells by secreting IL-2. Only CD3+, CD4+ hybrids responded to islet cells in our assay, and a large proportion of these hybrids were islet-cell reactive. T cell receptor (TCR) V beta element usage was heterogeneous in islet-reactive hybridomas.
- Published
- 1991
- Full Text
- View/download PDF