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Women experience numerous forms of discrimination in the workplace, both direct and indirect. Historically, bias against women was institutionalised by organisations and governments to keep women from pursuing long-term careers in science and remain in the home. Although changes to policy have occurred within Australia, societal perceptions around women in science have proven resistant to change, with discrimination continuing even today. Despite the barriers facing them, some women were able to break through and achieve impactful science, proving themselves vital members of the workforce and paving the way for future generations. Herein, we describe four such female chemist trailblazers, Isabel Joy Bear, Enid Plante, Catherine Anne Money and Annabelle Duncan, who each worked at the Council for Scientific and Industrial Research (CSIR) or the subsequent Commonwealth Scientific and Industrial Research Organisation (CSIRO) and helped to shape the face of chemistry in Australia in a male dominated environment. The stories of four trailblazing Australian female chemists, Isabel Joy Bear, Enid Plante, Catherine Anne Money and Annabelle Duncan, who broke barriers and paved the way for future generations. [ABSTRACT FROM AUTHOR]